Surface Modification of Polydimethylsiloxane with a Covalent Antithrombin-heparin Complex for Blood Contacting Applications
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The complexes were separated from free heparin by chromatography on antithrombin IJI-Sepharose or on DEAE-Sephadex and from unreacted antithrombin
High affinity heparin obtained by chromatography of clinical grade heparin on antithrombin 111-Sepharose was covalently coupled to antithrombin III using a three-step procedure: 1) introduction of free amino groups in the heparin molecule; 2) reaction of these amino groups with the bifunctional reagent tolylene2,4-diisothiocyanate; and 3) reaction of the remaining isothiocyanate group with amin...
متن کاملDesign of a new type of coating for the controlled release of heparin
Thrombus formation at the surface of blood contacting devices can be prevented by local release of heparin. Preferably, the release rate should be constant for prolonged periods of time. The minimum heparin release rate to achieve thromboresistance will be different for various applications and should therefore be adjustable. In this study a new type of heparin release system is presented which...
متن کاملEffect of a novel covalent antithrombin-heparin complex on thrombin generation on fetal distal lung epithelium.
Respiratory distress syndrome is characterized by fibrin deposition in the lung. Fibrin adversely affects surfactant function and stimulates proliferation of fibroblasts. There is evidence that these properties may be important to the development of bronchopulmonary dysplasia. Despite successful initial treatment of neonatal respiratory distress syndrome with surfactant, the incidence of bronch...
متن کاملCovalently bound conjugates of albumin and heparin: synthesis, fractionation and characterization.
Covalently bound conjugates of human serum albumin and heparin were prepared as compounds which could improve the blood-compatibility of polymer surfaces either by preadsorption or by covalent coupling of the conjugates onto blood contacting surfaces. The conjugates (10-16 weight % of heparin) were obtained by a condensation reaction between albumin and heparin using 1-ethyl-3-(dimethylaminopro...
متن کاملTHROMBOSIS AND HEMOSTASIS Antithrombin-heparin covalent complex reduces microemboli during cardiopulmonary bypass in a pig model
Transcranial Doppler-detected high-intensity transient signals (HITS) during cardiopulmonary bypass (CPB) surgery have been associated with postoperative neurocognitive dysfunction, suggesting microemboli in the brain could be a contributing factor. HITS occur despite administration of unfractionated heparin (UFH). This study was done to determine whether antithrombin-heparin covalent complex (...
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تاریخ انتشار 2013